Imèl nou pou plis enfòmasyon:
E:overseas@foregene.com
PCR dirèk se metòd anplifikasyon ADN ki soti dirèkteman nansan, tisi bèt,ke rat,selil,zòrèy rat,zèb pwason, ze pwason,fèy plant yo,grennoswa lòt echantiyon tisi byolojik orijinal san yo pa fè etap izolasyon ADN ak pirifikasyon.Teknik PCR dirèk ka redwi anpil tan eksperimantal, ak pri nan pwojè jenotip ak gwo kantite.Li bay tou yon pi bon opsyon lè yo fè fas ak defi yo nan anplifye yon ti kantite echantiyon kote etap pirifikasyon an kapab potansyèlman mennen nan pèt echantiyon.
PCR Mix FOREGENE a gen gwo rezistans estrès, akòz fòs FOREGENE patante Taq anzim (patant otorize: ZL20161034512.0 (Lachin), EP3450560 (Ewòp), PCT/CN2017/082154 (US), patant 6894926 (Japon)).Anzim Taq li a sèvi ak teknoloji recombination ADN pou rekonbine rejyon estrikti lyezon ADN nan pwoteyin asid nukleik asid ak ADN polymerase Taq ki reziste chalè pou konstwi yon ADN modèl ak afinite ogmante.Yon Taq cho-start amelyore ki konsève divès fonksyon nan ADN polymerase orijinal la. Anzim Taq sa a ka mare ak ADN modèl epi kòmanse sentèz ADN nan modèl konsantrasyon ki ba ak anviwònman konplèks.
For egzanp,itse osi fasil ke ajoute ouselil,plant (jenn fèy, rasin oswa grenn) oswa echantiyon bèt nan latanpon, epi ajoute inik lamèt melanje akespesifikprimè nan tib PCR ak kouri li nan yon sikilasyon tèmik.Efikasite twous se ideyal pou analiz echantiyon gwo echèl kote ekonomize tan trè enpòtan.
Se sistèm reyaksyon PCR ki gen anzim UNG ak dUTP chwazi selon kondisyon eksperimantal yo, ki ka efektivman evite polisyon ki te koze pa pwodwi anplifikasyon PCR yo.
PCR dirèk | Metòd tradisyonèl (RNA/ADN pirifikasyon + PCR konvansyonèl) | ||
Konsomasyon materyèl | Konsomasyon tisi | Mwens | Anpil |
Preparasyon modèl | Kalite materyèl | Pa gen fanm k'ap pile obligatwa | Manje echantiyon |
Operasyon | Kalite yon sèl-tib, 10min | Operasyon konplike, 1h | |
Flux | 1-96 | 1-24 | |
Reyaksyon PCR | Sistèm reyaksyon | Optimize 2 × PCR melanj | dNTP,MgCl2,10×PCR tanpon,Taq anzim |
01 Idantifikasyon mikwo-òganis patojèn
02 Jenetikman modifye idantifikasyon, jenotip
03 Multiplex PCR / SNP deteksyon / PCR-RFLP
04Sekans/klonaj
Non pwodwi | Espesifikasyon | Katalòg Nimewo | Kondisyon Depo |
QuickEasyTMTwous Cell Direct RT-qPCR–SYBR Green I/Taqman | 200T | DRT-01011 /DRT-01021 | Pati I 4℃,Pati II -20℃ |
1000T | DRT-01012 /DRT-01022 |
Non pwodwi | Espesifikasyon | Katalòg Nimewo | Kondisyon Depo |
San SuperDirectTMKit PCR (EDTA) | 200 × 20μl rxns | TP-04111 | -20℃ |
2000 × 20μl rxns | TP-04113 | ||
San SuperDirectTMKit PCR (EDTA) -UNG | 200 × 20μl rxns | TP-04121 | -20℃ |
2000 × 20μl rxns | TP-04123 | ||
San SuperDirectTMKit PCR (eparin) | 200 × 20μl rxns | TP-04211 | -20℃ |
2000 × 20μl rxns | TP-04213 | ||
San SuperDirectTMPCR Twous (Heparin) -UNG | 200 × 20μl rxns | TP-04221 | -20℃ |
2000 × 20μl rxns | TP-04223 |
Non pwodwi | Espesifikasyon | Katalòg Nimewo | Kondisyon Depo |
Animal Tissus Direct PCR Twous | 200 × 20μl rxns | TP-01111 | Pati I 4℃,Pati II -20℃ |
2000 × 20μl rxns | TP-01113 | ||
Animal Tissus Direct PCR Twous (65 ℃ lysis) -UNG | 200 × 20μl rxns | TP-01121 | Pati I 4℃,Pati II -20℃ |
2000 × 20μl rxns | TP-01123 | ||
Mouse Tail SuperDirectTM PCR Twous (liz tanperati chanm) | 200 × 20μl rxns | TP-01311 | Pati I 4℃,Pati II -20℃ |
2000 × 20μl rxns | TP-01313 | ||
Mouse Tail Direct PCR Twous (65 ℃ liz) | 200 × 20μl rxns | TP-01331 | Pati I 4℃,Pati II -20℃ |
2000 × 20μl rxns | TP-01333 | ||
Mouse Tail Direct PCR Kit (65 ℃ lysis) -UNG | 200 × 20μl rxns | TP-01341 | Pati I 4℃,Pati II -20℃ |
2000 × 20μl rxns | TP-01343 | ||
Zebra Fish Direct PCR Twous (65 ℃ liz) | 200 × 20μl rxns | TP-01411 | Pati I 4℃,Pati II -20℃ |
2000 × 20μl rxns | TP-01413 | ||
Zebra Fish Direct PCR Kit (65 ℃ lysis) -UNG | 200 × 20μl rxns | TP-01421 | Pati I 4℃,Pati II -20℃ |
2000 × 20μl rxns | TP-01423 |
Non pwodwi | Espesifikasyon | Katalòg Nimewo | Kondisyon Depo |
Plant Leaf Direct PCR Twous | 200 × 20μl rxns | TP-02111 | Pati I 4℃,Pati II -20℃ |
2000 × 20μl rxns | TP-02113 | ||
Plant Leaf Direct PCR Kit -UNG | 200 × 20μl rxns | TP-02121 | Pati I 4℃,Pati II -20℃ |
2000 × 20μl rxns | TP-02123 | ||
Plant Leaf Direct PCR Plus Twous (Plant polisakarid polifenol) | 200 × 20μl rxns | TP-02131 | Pati I 4℃,Pati II -20℃ |
2000 × 20μl rxns | TP-02133 | ||
Plant Leaf Direct PCR Plus Kit -UNG(Plant polisakarid polifenol) | 200 × 20μl rxns | TP-02141 | Pati I 4℃,Pati II -20℃ |
2000 × 20μl rxns | TP-02143 | ||
Plant Seed Direct PCR Twous I | 200 × 20μl rxns | TP-03111 | Pati I 4℃,Pati II -20℃ |
2000 × 20μl rxns | TP-03113 | ||
Plant Seed Direct PCR Kit I -UNG | 200 × 20μl rxns | TP-03121 | Pati I 4℃,Pati II -20℃ |
2000 × 20μl rxns | TP-03123 | ||
Plant Seed Direct PCR Twous II | 200 × 20μl rxns | TP-03131 | Pati I 4℃,Pati II -20℃ |
2000 × 20μl rxns | TP-03133 | ||
Plant Seed Direct PCR Kit II -UNG | 200 × 20μl rxns | TP-03141 | Pati I 4℃,Pati II -20℃ |
2000 × 20μl rxns | TP-03143 | ||
Plant Seed Direct PCR Plus Twous I | 200 × 20μl rxns | TP-03151 | Pati I 4℃,Pati II -20℃ |
2000 × 20μl rxns | TP-03153 | ||
Plant Seed Direct PCR Plus Kit I -UNG | 200 × 20μl rxns | TP-03161 | Pati I 4℃,Pati II -20℃ |
2000 × 20μl rxns | TP-03163 | ||
Plant Seed Direct PCR Plus Twous II | 200 × 20μl rxns | TP-03171 | Pati I 4℃,Pati II -20℃ |
2000 × 20μl rxns | TP-03173 | ||
Plant Seed Direct PCR Plus Kit II -UNG | 200 × 20μl rxns | TP-03181 | Pati I 4℃,Pati II -20℃ |
2000 × 20μl rxns | TP-03183 |
* Twous RT-PCR asid nikleik Covid, pou ORF1ab, N, E,S, ak lòt mutan, tankou Delta variant, elatriye.
96Rxns/twous
Benefis:
-Fè tès la nan apeprè 1 èdtan
-Pa bezwen fè ekstraksyon RNA ak izòlman
-Segondè espesifik ak sansiblite
Imèl nou pou plis enfòmasyon:
E:overseas@foregene.com