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PCR dirèk

Ki sa ki se Direct PCR?

PCR dirèk se metòd anplifikasyon ADN ki soti dirèkteman nansan, tisi bèt,ke rat,selil,zòrèy rat,zèb pwason, ze pwason,fèy plant yo,grennoswa lòt echantiyon tisi byolojik orijinal san yo pa fè etap izolasyon ADN ak pirifikasyon.Teknik PCR dirèk ka redwi anpil tan eksperimantal, ak pri nan pwojè jenotip ak gwo kantite.Li bay tou yon pi bon opsyon lè yo fè fas ak defi yo nan anplifye yon ti kantite echantiyon kote etap pirifikasyon an kapab potansyèlman mennen nan pèt echantiyon.

PCR Mix FOREGENE a gen gwo rezistans estrès, akòz fòs FOREGENE patante Taq anzim (patant otorize: ZL20161034512.0 (Lachin), EP3450560 (Ewòp), PCT/CN2017/082154 (US), patant 6894926 (Japon)).Anzim Taq li a sèvi ak teknoloji recombination ADN pou rekonbine rejyon estrikti lyezon ADN nan pwoteyin asid nukleik asid ak ADN polymerase Taq ki reziste chalè pou konstwi yon ADN modèl ak afinite ogmante.Yon Taq cho-start amelyore ki konsève divès fonksyon nan ADN polymerase orijinal la. Anzim Taq sa a ka mare ak ADN modèl epi kòmanse sentèz ADN nan modèl konsantrasyon ki ba ak anviwònman konplèks.

For egzanp,itse osi fasil ke ajoute ouselil,plant (jenn fèy, rasin oswa grenn) oswa echantiyon bèt nan latanpon, epi ajoute inik lamèt melanje akespesifikprimè nan tib PCR ak kouri li nan yon sikilasyon tèmik.Efikasite twous se ideyal pou analiz echantiyon gwo echèl kote ekonomize tan trè enpòtan.

Se sistèm reyaksyon PCR ki gen anzim UNG ak dUTP chwazi selon kondisyon eksperimantal yo, ki ka efektivman evite polisyon ki te koze pa pwodwi anplifikasyon PCR yo.

Avantaj ak aplikasyon PCR dirèk

asdada (5)
asdada (1)
Avantaj PCR dirèk
Aplikasyon
Avantaj PCR dirèk
    PCR dirèk Metòd tradisyonèl
(RNA/ADN pirifikasyon + PCR konvansyonèl)
Konsomasyon materyèl Konsomasyon tisi Mwens Anpil
Preparasyon modèl Kalite materyèl Pa gen fanm k'ap pile obligatwa Manje echantiyon
Operasyon Kalite yon sèl-tib, 10min Operasyon konplike, 1h
Flux 1-96 1-24
Reyaksyon PCR Sistèm reyaksyon Optimize 2 × PCR melanj dNTP,MgCl2,10×PCR tanpon,Taq anzim
Aplikasyon

01 Idantifikasyon mikwo-òganis patojèn

02 Jenetikman modifye idantifikasyon, jenotip

03 Multiplex PCR / SNP deteksyon / PCR-RFLP

04Sekans/klonaj

Pwodwi Foregene Direct PCR kits

Twous RT-qPCR dirèk selil yo
Twous PCR san dirèk
Animal Direct PCR Twous
Plant Direct PCR Kits
Twous IVD
Twous RT-qPCR dirèk selil yo
Non pwodwi Espesifikasyon Katalòg
Nimewo
Kondisyon Depo
QuickEasyTMTwous Cell Direct RT-qPCR–SYBR Green I/Taqman 200T DRT-01011
/DRT-01021
Pati I 4℃,Pati II -20℃
1000T DRT-01012
/DRT-01022
Twous PCR san dirèk
Non pwodwi Espesifikasyon Katalòg
Nimewo
Kondisyon Depo
San SuperDirectTMKit PCR (EDTA) 200 × 20μl rxns TP-04111 -20℃
2000 × 20μl rxns TP-04113
San SuperDirectTMKit PCR (EDTA) -UNG 200 × 20μl rxns TP-04121 -20℃
2000 × 20μl rxns TP-04123
San SuperDirectTMKit PCR (eparin) 200 × 20μl rxns TP-04211 -20℃
2000 × 20μl rxns TP-04213
San SuperDirectTMPCR Twous (Heparin) -UNG 200 × 20μl rxns TP-04221 -20℃
2000 × 20μl rxns TP-04223
Animal Direct PCR Twous
Non pwodwi Espesifikasyon Katalòg
Nimewo
Kondisyon Depo
Animal Tissus Direct PCR Twous 200 × 20μl rxns TP-01111 Pati I 4℃,Pati II -20℃
2000 × 20μl rxns TP-01113
Animal Tissus Direct PCR Twous (65 ℃ lysis) -UNG 200 × 20μl rxns TP-01121 Pati I 4℃,Pati II -20℃
2000 × 20μl rxns TP-01123
Mouse Tail SuperDirectTM PCR Twous (liz tanperati chanm) 200 × 20μl rxns TP-01311 Pati I 4℃,Pati II -20℃
2000 × 20μl rxns TP-01313  
Mouse Tail Direct PCR Twous
(65 ℃ liz)
200 × 20μl rxns TP-01331 Pati I 4℃,Pati II -20℃
2000 × 20μl rxns TP-01333
Mouse Tail Direct PCR Kit (65 ℃ lysis) -UNG 200 × 20μl rxns TP-01341 Pati I 4℃,Pati II -20℃
2000 × 20μl rxns TP-01343
Zebra Fish Direct PCR Twous (65 ℃ liz) 200 × 20μl rxns TP-01411 Pati I 4℃,Pati II -20℃
2000 × 20μl rxns TP-01413
Zebra Fish Direct PCR Kit (65 ℃ lysis) -UNG 200 × 20μl rxns TP-01421 Pati I 4℃,Pati II -20℃
2000 × 20μl rxns TP-01423
Plant Direct PCR Kits
Non pwodwi Espesifikasyon Katalòg
Nimewo
Kondisyon Depo
Plant Leaf Direct PCR Twous 200 × 20μl rxns TP-02111 Pati I 4℃,Pati II -20℃
2000 × 20μl rxns TP-02113
Plant Leaf Direct PCR Kit -UNG 200 × 20μl rxns TP-02121 Pati I 4℃,Pati II -20℃
2000 × 20μl rxns TP-02123
Plant Leaf Direct PCR Plus Twous
(Plant polisakarid polifenol)
200 × 20μl rxns TP-02131 Pati I 4℃,Pati II -20℃
2000 × 20μl rxns TP-02133
Plant Leaf Direct PCR Plus Kit -UNG(Plant polisakarid polifenol) 200 × 20μl rxns TP-02141 Pati I 4℃,Pati II -20℃
2000 × 20μl rxns TP-02143
Plant Seed Direct PCR Twous I 200 × 20μl rxns TP-03111 Pati I 4℃,Pati II -20℃
2000 × 20μl rxns TP-03113
Plant Seed Direct PCR Kit I -UNG 200 × 20μl rxns TP-03121 Pati I 4℃,Pati II -20℃
2000 × 20μl rxns TP-03123
Plant Seed Direct PCR Twous II 200 × 20μl rxns TP-03131 Pati I 4℃,Pati II -20℃
2000 × 20μl rxns TP-03133
Plant Seed Direct PCR Kit II -UNG 200 × 20μl rxns TP-03141 Pati I 4℃,Pati II -20℃
2000 × 20μl rxns TP-03143
Plant Seed Direct PCR Plus Twous I 200 × 20μl rxns TP-03151 Pati I 4℃,Pati II -20℃
2000 × 20μl rxns TP-03153
Plant Seed Direct PCR Plus Kit I -UNG 200 × 20μl rxns TP-03161 Pati I 4℃,Pati II -20℃
2000 × 20μl rxns TP-03163
Plant Seed Direct PCR Plus Twous II 200 × 20μl rxns TP-03171 Pati I 4℃,Pati II -20℃
2000 × 20μl rxns TP-03173
Plant Seed Direct PCR Plus Kit II -UNG 200 × 20μl rxns TP-03181 Pati I 4℃,Pati II -20℃
2000 × 20μl rxns TP-03183

 

Twous IVD

* Twous RT-PCR asid nikleik Covid, pou ORF1ab, N, E,S, ak lòt mutan, tankou Delta variant, elatriye.

96Rxns/twous

Benefis:

-Fè tès la nan apeprè 1 èdtan

-Pa bezwen fè ekstraksyon RNA ak izòlman

-Segondè espesifik ak sansiblite

Detay kit tès Covid klike isit la