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Dirèk RT-qPCR seri

PCR dirèk

Ki sa ki se Direct PCR?

PCR dirèk se metòd anplifikasyon ADN ki soti dirèkteman nan san, tisi bèt, ke rat, selil, zòrèy rat, pwason zèb, ze pwason, fèy plant, grenn oswa lòt echantiyon tisi byolojik orijinal san yo pa fè etap izolasyon ADN ak pirifikasyon.Teknik PCR dirèk ka redwi anpil tan eksperimantal, ak pri nan pwojè jenotip ak gwo kantite.Li bay tou yon pi bon opsyon lè yo fè fas ak defi yo nan anplifye yon ti kantite echantiyon kote etap pirifikasyon an kapab potansyèlman mennen nan pèt echantiyon.

PCR Mix FOREGENE a gen gwo rezistans estrès, akòz fòs FOREGENE patante Taq anzim (patant otorize: ZL20161034512.0 (Lachin), EP3450560 (Ewòp), PCT/CN2017/082154 (US), patant 6894926 (Japon)).Anzim Taq li a sèvi ak teknoloji recombination ADN pou rekonbine rejyon estrikti lyezon ADN nan pwoteyin asid nukleik asid ak ADN polymerase Taq ki reziste chalè pou konstwi yon ADN modèl ak afinite ogmante.Yon Taq cho-start amelyore ki konsève divès fonksyon nan ADN polymerase orijinal la. Anzim Taq sa a ka mare ak ADN modèl epi kòmanse sentèz ADN nan modèl konsantrasyon ki ba ak anviwònman konplèks.

Pou egzanp, li fasil menm jan ak ajoute selil ou, plant (jenn fèy, rasin oswa grenn) oswa echantiyon bèt nan tanpon an, epi ajoute melanj inik mèt la ak primè espesifik nan tib PCR epi kouri li nan yon sikilasyon tèmik.Efikasite twous se ideyal pou analiz echantiyon gwo echèl kote ekonomize tan trè enpòtan.

Se sistèm reyaksyon PCR ki gen anzim UNG ak dUTP chwazi selon kondisyon eksperimantal yo, ki ka efektivman evite polisyon ki te koze pa pwodwi anplifikasyon PCR yo.

asdada (5)
asdada (1)

Avantaj nanDPCR dirèk

 

 

PCR dirèk

Metòd tradisyonèl

(RNA pirifikasyon + PCR konvansyonèl)

Konsomasyon materyèl

Konsomasyon tisi

Mwens

Anpil

Preparasyon modèl

Kalite materyèl

Pa gen fanm k'ap pile obligatwa

Manje echantiyon

Operasyon

Kalite yon sèl-tib, 10min

operasyon konplike, 1h

Flux

1-96

1-24

Reyaksyon PCR

sistèm reyaksyon

Optimize 2 × PCR melanj

Dntp, MgCl2,10 × PCR tanpon, Taq anzim,

Aaplikasyon

01 Idantifikasyon mikwo-òganis patojèn

02 Jenetikman modifye idantifikasyon, jenotip

03 Multiplex PCR / SNP deteksyon / PCR-RFLP

04Sekans/klonaj

Seri pwodwi

1

Seri

Non pwodwi

Espesifikasyon

Nimewo Katalòg

Kondisyon Depo

Dirèk RT-qPCR Seri

QuickEasyTMTwous Cell Direct RT-qPCR–SYBR Green I

200T

DRT-01011

Pati I 4℃,Pati II -20℃

1000T

DRT-01012

 

2

Seri

Non pwodwi

Espesifikasyon

Nimewo Katalòg

Kondisyon Depo

 

 

 

Seri PCR san dirèk

San SuperDirectTMKit PCR (EDTA)

200 × 20μl rxns

TP-04111

-20℃

2000 × 20μl rxns

TP-04113

San SuperDirectTMKit PCR (EDTA)-UNG

200 × 20μl rxns

TP-04121

-20℃

2000 × 20μl rxns

TP-04123

San SuperDirectTMKit PCR
(Eparin)

200 × 20μl rxns

TP-04211

-20℃

2000 × 20μl rxns

TP-04213

San SuperDirectTMPCR Twous (Heparin) -UNG

200 × 20μl rxns

TP-04221

-20℃

2000 × 20μl rxns

TP-04223

3
4

Seri

Non pwodwi

Espesifikasyon

Nimewo Katalòg

Kondisyon Depo

Animal Direct PCR Seri

Tisi Animal Direct PCR Twous (65 ℃ liz)

200 × 20μl rxns

TP-01111

Pati I 4℃,Pati II -20℃

2000 × 20μl rxns

TP-01113

Animal Tissus Direct PCR Twous (65 ℃ lysis) -UNG

200 × 20μl rxns

TP-01121

Pati I 4℃,Pati II -20℃

2000 × 20μl rxns

TP-01123

Sourit ke SuperDirectTMKit PCR
(liz tanperati chanm)

200 × 20μl rxns

TP-01311

Pati I 4℃,Pati II -20℃

2000 × 20μl rxns

TP-01313

Mouse Tail Direct PCR Twous (65 ℃ liz)

200 × 20μl rxns

TP-01331

Pati I 4℃,Pati II -20℃

2000 × 20μl rxns

TP-01333

Mouse Tail Direct PCR Kit (65 ℃ lysis) - UNG

200 × 20μl rxns

TP-01341

Pati I 4℃,Pati II -20℃

2000 × 20μl rxns

TP-01343

Zebra Fish Direct PCR Twous (65 ℃ liz)

200 × 20μl rxns

TP-01411

Pati I 4℃,Pati II -20℃

2000 × 20μl rxns

TP-01413

Zebra Fish Direct PCR Kit (65 ℃ lysis) - UNG

200 × 20μl rxns

TP-01421

Pati I 4℃,Pati II -20℃

2000 × 20μl rxns

TP-01423

 

5
6

Seri

Non pwodwi

Espesifikasyon

Nimewo Katalòg

Kondisyon Depo

Plant Direct PCR Seri

Plant Leaf Direct PCR Twous

200 × 20μl rxns

TP-02111

Pati I 4℃,Pati II -20℃

2000 × 20μl rxns

TP-02113

Plant Leaf Direct PCR Kit -UNG

200 × 20μl rxns

TP-02121

Pati I 4℃,Pati II -20℃

2000 × 20μl rxns

TP-02123

Plant Leaf Direct PCR Plus Twous
(Plant polisakarid polifenol)

200 × 20μl rxns

TP-02131

Pati I 4℃,Pati II -20℃

2000 × 20μl rxns

TP-02133

Plant Leaf Direct PCR Plus Kit -UNG
(Plant polisakarid polifenol)

200 × 20μl rxns

TP-02141

Pati I 4℃,Pati II -20℃

2000 × 20μl rxns

TP-02143

Plant Seed Direct PCR Twous I

200 × 20μl rxns

TP-03111

Pati I 4℃,Pati II -20℃

2000 × 20μl rxns

TP-03113

Plant Seed Direct PCR Kit I -UNG

200 × 20μl rxns

TP-03121

Pati I 4℃,Pati II -20℃

2000 × 20μl rxns

TP-03123

Plant Seed Direct PCR Twous II

200 × 20μl rxns

TP-03131

Pati I 4℃,Pati II -20℃

2000 × 20μl rxns

TP-03133

Plant Seed Direct PCR Kit II -UNG

200 × 20μl rxns

TP-03141

Pati I 4℃,Pati II -20℃

2000 × 20μl rxns

TP-03143

Plant Seed Direct PCR Plus Kit I (plant polisakarid polifenol)

200 × 20μl rxns

TP-03151

Pati I 4℃,Pati II -20℃

2000 × 20μl rxns

TP-03153

Plant Seed Direct PCR Plus Kit I - UNG (plant polisakarid polifenol)

200 × 20μl rxns

TP-03161

Pati I 4℃,Pati II -20℃

2000 × 20μl rxns

TP-03163

Plant Seed Direct PCR Plus Twous II

200 × 20μl rxns

TP-03171

Pati I 4℃,Pati II -20℃

2000 × 20μl rxns

TP-03173

Plant Seed Direct PCR Plus Kit II - UNG (plant polisakarid polifenol)

200 × 20μl rxns

TP-03181

Pati I 4℃,Pati II -20℃

2000 × 20μl rxns

TP-03183